GAPDH as a Control Gene to Estimate Genome Copy Number in Great Tits, with Cross-Amplification in Blue Tits

Ardea

Els Atema¹  Simon Verhulst³  Kees van Oers² 

[1] 1 Department of Behavioural Biology, Centre for Life Sciences, University of Groningen, P.O. Box 11103, 9700 CC Groningen, The Netherlands 2 Department of Animal Ecology, Netherlands Institute of Ecology (NIOO-KNAW), P.O. Box 50, 6700 AB Wageningen, The Netherlands;2 Department of Animal Ecology, Netherlands Institute of Ecology (NIOO-KNAW), P.O. Box 50, 6700 AB Wageningen, The Netherlands;1 Department of Behavioural Biology, Centre for Life Sciences, University of Groningen, P.O. Box 11103, 9700 CC Groningen, The Netherlands

关键词: Passerine;   

DOI  :  

学科分类：动物科学

来源: Nederlandse Ornithologische Unie/Netherlands Ornithological Union

【 摘 要 】

Estimatingthenumberofgenomecopiesinatissuesamplecanservevariouspurposes.Forexample,suchanestimateservesasascalingvariablewhenmeasuringtelomereswithquantitativePCR.Wedescribetheprimerdevelopmentandevaluationfortheglyceraldehyde-3-phosphatedehydrogenase(GAPDH)geneintheGreatTitParusmajor,asacontrolgenetoestimategenomecopynumber.Wedemonstratespecificamplificationwithnegligiblevariationin48GreatTitsandcross-amplificationin53BlueTitsCyanistescaeruleus.WeconcludethisprimersettobereliableforamplificationofGAPDHasareferencegeneforquantitativePCRanalysisinGreatandBlueTits.

【 授权许可】

Unknown