Fluid Dynamics and Electrical Detection of λDNA in Electrode-Embedded Nanochannels

Journal of Biomechanical Science and Engineering

Satoshi UEHARA¹  Masateru TANIGUCHI²  Kentaro DOI¹  Makusu TSUTSUI²  Satoyuki KAWANO¹  Tomoji KAWAI² 

[1] Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University;The Institute of Scientific and Industrial Research (ISIR), Osaka University

关键词: Nanofluidics;    Biomacromorecule;    Electrophoresis;    DNA Sequencer;    Single Molecule Observation;   

DOI  :  10.1299/jbse.8.244

来源: Japan Society of Mechanical Engineers

【 摘 要 】

References(23)Cited-By(3)In the present study, we address theoretical approaches for the experimental results to investigate the flow dynamics of λDNA through a nanochannel in which two nanoelectrodes are integrated. In order to elucidate the relationship between the longitudinal ionic current and the electrophoresis of λDNA in the specific micro/nanofluidics, we develop a theoretical model for the macroscopic fluid dynamics in a Lagrangian framework. The measured current change associated with a single molecule translocation through the channel is explained by the principle of the Coulter counter that allowed to predict the conformation of λDNA. We also analyze the local velocity of λDNA passing through a nanoscaled confined channel. A result from the model is in considerable agreement with the experimental observations for the electrophoretic flow of λDNA. The basic knowledge obtained here may be useful in developing electrical methods for controlling the electrophoretic velocity of single-molecule DNA for realizing the nanopore sequencer.

【 授权许可】

Unknown