Surface Morphology and Stiffness of Cartilage-Like Tissue Repaired with a Scaffold-Free Tissue Engineered Construct

Journal of Biomechanical Science and Engineering

Kazunori SHIMOMURA¹  Wataru ANDO¹  Hiromichi FUJIE²  Ryosuke NANSAI³  Takuya SUZUKI³  Norimasa NAKAMURA¹ 

[1] Department of Orthopaedics, Osaka University Graduate School of Medicine;Faculty of System Design, Tokyo Metropolitan University;Biomechanics Laboratory, Graduate School of Engineering, Kogakuin University

关键词: Cartilage Repair;    Mesenchymal Stem Cell;    Tissue Engineered Construct (TEC);    Scaffold Free;    Atomic Force Microscope (AFM);    Stiffness;    Surface Morphology;   

DOI  :  10.1299/jbse.6.40

来源: Japan Society of Mechanical Engineers

【 摘 要 】

References(20)Cited-By(5)We have been developing a new tissue engineering technique for cartilage repair using a scaffold-free tissue engineered construct (TEC) bio-synthesized from synovium-derived mesenchymal stem cells (MSCs). A round-shaped chondral defect of 8.5 mm in diameter and 2.0 mm in depth created on the medial condyle of immature (4-month-old) and mature (12-month-old) porcine femur was filled with the TEC. Six months after surgery, a cylindrically shaped specimen of 4 mm in diameter and 4-5 mm in depth was extracted. Stiffness measurements were carried out on the specimen using an AFM after the surface image of the specimen was obtained. The TEC-treated tissues exhibited more irregular surface as compared with normal cartilage, regardless of animal maturity. The stiffness of the superficial layer of the TEC-treated tissue was significantly lower than those of the normal cartilage, indicating 6.8±2.3 mN/m in the immature group and 8.8±2.3 mN/m in the mature group. Histological observation indicated that the defects were repaired with a hyaline cartilage-like tissue with positive Safranine O staining, regardless of animal maturity. However, the superficial layer of the repaired tissues was negatively stained with Safranine O. The present study suggests that the recovery of the superficial layer is delayed in both immature and mature animals although the treatment with TEC enhances the repair of partial chondral defects.

【 授权许可】

Unknown