Effect of Protease-Activated Receptor-2 Deficiency on Allergic Dermatitis in the Mouse Ear

The Japanese Journal of Pharmacology

Gary D. Hunter¹  Naohiro Saito³  Jiro Matsumoto³  Robin Plevin¹  Masaki Tamiya³  Toshiaki Takizawa³  Stephen E. Meek²  Yasushi Wada³  Andrew J.H. Smith²  Junichi Kawagoe³  Mikio Fujii³  Toru Kanke³ 

[1] Department of Physiology and Pharmacology, University of Strathclyde, Strathclyde Institute for Biomedical Sciences;Centre for Genome Research, The University of Edinburgh;Tokyo Research Laboratories, Pharmaceutical Division, Kowa Company Ltd.

关键词: Protease-activated receptor-2;    Knockout mice;    Allergic dermatitis;    Contact hypersensitivity;    Passive cutaneous anaphylaxis;   

DOI  :  10.1254/jjp.88.77

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(49)Cited-By(35)To investigate the involvement of protease-activated receptor-2 (PAR-2) in allergic dermatitis, we generated PAR-2-deficient (PAR-2−/−) mice. Ear thickness, contact hypersensitivity (CH) induced by topical application of picryl chloride (PC) or oxazolone (Ox) after sensitization, and vascular permeability after ear passive cutaneous anaphylaxis (PCA) were compared between wild-type (WT) and PAR-2−/− mice. Ear thickness was almost the same in untreated WT and PAR-2−/− mice. Topical application of PC or Ox thickened the ears at 6, 24 and 48 h after challenge with a peak at 24 h in WT mice. In PAR-2−/− mice, the ear swelling induced by both PC and Ox was suppressed at every time point, and significant inhibition was found at 24 h in PC-induced CH and at 24 and 48 h in Ox-induced CH. Histopathological observation of the ears at 24 h after challenge revealed that PC- or Ox-induced ear edema and infiltration of inflammatory cells in WT mice were greatly attenuated in PAR-2−/− mice. The vascular permeability in the ears after PCA was not different between WT and PAR-2−/− mice. These results strongly suggest that PAR-2 plays a crucial role in type IV allergic dermatitis but not in type I allergic dermatitis.

【 授权许可】

Unknown