Guinea Pig Histamine N-Methyltransferase: cDNA Cloning and mRNA Distribution

The Japanese Journal of Pharmacology

Nobuyuki Terada²  Junichi Kitanaka¹  Motohiko Takemura¹  Nobue Kitanaka¹  Tohru Tsujimura²  Michiko Kakihana² 

[1] Pharmacology;Pathology I, Hyogo College of Medicine

关键词: Histamine N-methyltransferase;    mRNA;    Localization;   

DOI  :  10.1254/jjp.85.105

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(6)We report here the isolation of histamine N-methyltransferase (HMT) cDNA from the guinea pig brain by the polymerase chain reaction on the basis of nucleotide sequences of rat and human counterparts. Guinea pig HMT consists of 292 amino acids, with homologies of 75.6% and 79.1% to rat and human HMT, respectively. Northern blotting analysis indicated that the 1.6-kb guinea pig HMT transcript was expressed at various levels in different tissues at the following relative abundance: jejunum, brain > lung, spleen, stomach > liver, kidney. HMT mRNA localized throughout the jejunum, and it was mainly expressed in epithelial cells and in Auerbach's plexus.

【 授权许可】

Unknown