The Mechanism of Maitotoxin-Induced Elevation of the Cytosolic Free Calcium Level in Rat Cerebrocortical Synaptosomes

The Japanese Journal of Pharmacology

Masakazu Nishimura¹  Eiki Satoh¹  Toshiaki Ishii¹ 

[1] Department of Pharmacology, University of Obihiro School of Veterinary Medicine

关键词: Maitotoxin;    Synaptosomes;    Cytosolic free calcium;   

DOI  :  10.1254/jjp.85.98

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(5)The present study was conducted to elucidate the mechanism of the maitotoxin (MTX)-induced increase in intrasynaptosomal free calcium level ([Ca2+]i). The MTX (1 ng/ml)-induced increase in [Ca2+]i was partially inhibited by the omission of extracellular Ca2+ (Ca2+e) or the addition of verapamil, but not by adding nifedipine, ω-agatoxin IVA, ω-conotoxin GVIA and ω-conotoxin MVIIC. An increase in [Ca2+]i in the absence of Ca2+e was sensitive to procaine, TMB-8, genistein and verapamil, but not to ryanodine and U-73122. These results may suggest that MTX increases [Ca2+]i by stimulating Ca2+ entry through voltage-independent nonselective cation channels and Ca2+ release from stores through a phospholipase C-γ1-mediated pathway in rat cerebrocortical synaptosomes.

【 授权许可】

Unknown