Effect of APGW-Amide on [Ca2+]i in Rat Pheochromocytoma PC12 Cells

The Japanese Journal of Pharmacology

Yoshinori Nozawa¹  Xiao Yan Han²  Yifa Jiang³  Kenichi Matsunami²  Yuzuru Ito¹ 

[1] Department of Biochemistry, Gifu University School of Medicine, Gifu 500-8705, Japan;Department of Neurophysiology, Institute of Equilibrium Research, Gifu University School of Medicine, Gifu 500-8705, Japan;Virtual System Laboratory, Gifu University, Gifu 501-1193, Japan

关键词: APGW-amide;    PC12 cell;    L-type Ca2+ channel;   

DOI  :  10.1254/jjp.84.355

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)In order to determine whether Ala-Pro-Gly-Try-NH2 (APGW-amide) could affect mammalian excitable cells, we investigated the effect of APGW-amide in PC12 cells. APGW-amide caused a rapid [Ca2+]i elevation, which was completely prevented by elimination of extracellular Ca2+ with EGTA and inhibited by two L-type Ca2+ channel blockers. [Ca2+]i elevation was also blocked by a specific PKC inhibitor and prolonged pretreatment of cells with PMA. These results indicate that APGW-amide elevates [Ca2+]i in PC12 cells, possibly by Ca2+ influx via L-type Ca2+ channel activated by PKC.

【 授权许可】

Unknown