Intracellular Ca2+ Increase in Neuro-2A Cells and Rat Astrocytes Following Stimulation of Bradykinin B2 Receptor

The Japanese Journal of Pharmacology

Akinori Ueno¹  Yuri Ikeda¹  Hiroaki Naraba¹  Norio Matsuki²  Sachiko Oh-ishi¹ 

[1] Department of Pharmacology,School of Pharmaceutical Sciences,Kitasato University,5-9-1 Shirokane,Minato-ku,Tokyo 108-8641,Japan;Department of Pharmacology,Faculty of Pharmaceutical Sciences,Tokyo University,7-3-1 Hongo,Bunkyo-ku,Tokyo 113-0033,Japan

关键词: Bradykinin;    Neuro-2A;    Astrocyte;    Intracellular Ca2+;    Bradykinin B2 receptor;   

DOI  :  10.1254/jjp.84.140

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(20)Cited-By(9)Murine neuroblastoma cell line Neuro−2A cells and rat brain astrocytes showed a dose−dependent increase in intracellular Ca2+ in response to bradykinin, when assessed by a single cell image analyzing system.The Ca2+ increase in Neuro−2A cells by bradykinin was also examined by a suspension fluorescent assay using fura−2 loading.The Ca2+ increase in both cases was suppressed by a bradykinin B2 receptor antagonist, Hoe 140, but not by a B1 receptor antagonist, des−Arg−Hoe 140, suggesting that the effect occurred via specific B2 receptor activation.RT−PCR for bradykinin B2 receptor mRNA showed that both Neuro−2A cells and the astrocytes expressed B2 receptor mRNA.Binding of [3H]bradykinin to Neuro−2A cells was assessed, and a specific binding constant of 0.75 nM was determined.Furthermore, the increase in [Ca2+]i by bradykinin could be caused by a release of Ca2+ from storage sites in the endoplasmic reticulum, since thapsigargin and U−73122 attenuated the effect of bradykinin in Neuro−2A as well as in astrocytes.These results indicate that both astrocytes and neuroblastoma Neuro−2A cells stimulated by bradykinin could express a bradykinin B2 receptor−mediated intracellular Ca2+ increase leading to signal transduction.

【 授权许可】

Unknown