Enhancement of the Serotonin-Mediated Acetylcholine Release by Repeated Desmethylimipramine Treatment in the Hippocampus of Freely Moving Rats

The Japanese Journal of Pharmacology

Kazuko Fujimoto¹  Katsuhiko Nakai¹  Shigeru Ohba¹  Takeshi Fujii¹  Koichiro Kawashima¹  Takeshi Suzuki¹ 

[1] Department of Pharmacology, Kyoritsu College of Pharmacy

关键词: Acetylcholine;    Desmethylimipramine;    Hippocampus;    Microdialysis;    Serotonin (5-HT);   

DOI  :  10.1254/jjp.80.303

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(37)Cited-By(3)A possible involvement of serotonin-mediated cholinergic activation in the antidepressant effect of desmethylimipramine (DMI) was investigated by determination of the effects of a single or repeated DMI administration on acetylcholine (ACh) release in the hippocampus using an in vivo microdialysis technique and a radioimmunoassay for ACh. Rats were administered DMI (10 mg/kg, i.p.) acutely or repeatedly for 21 days. A single or repeated DMI administration did not cause any significant effects on the basal ACh release compared with the respective controls. Atropine perfusion in the acutely DMI-treated or control rats increased the ACh release to the same degree. In repeatedly DMI-treated rats, serotonin (5-HT) (1 to 10 μM) perfusion enhanced significantly the ACh release. However, 5-HT in acutely DMI-treated rats enhanced significantly the ACh release only at 10 μM. 5-HT did not cause any changes in ACh release in control rats. Hippocampal 5-HT content of acutely DMI-treated rats was significantly higher than that of saline-treated control rats, while no difference was observed between the repeatedly DMI- and saline-treated rats. These findings suggest, for the first time, that DMI induced a facilitation of cholinergic neurotransmission in the rat hippocampus through the activation of 5-HT-receptor function.

【 授权许可】

Unknown