Pancreatic Enzyme-Induced Pancreatitis and Systemic Complications in Rats

The Japanese Journal of Pharmacology

Keiji Miyata¹  Mayumi Yamano¹  Toshimitsu Yamada¹ 

[1] Pharmacology Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd.

关键词: Acute pancreatitis;    Pancreatic enzyme;    Pulmonary dysfunction;    Trypsin;    Elastase;   

DOI  :  10.1254/jjp.77.185

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(27)Cited-By(4)Effects of retrograde injection into the pancreatic duct and intravenous infusion of pancreatic enzymes and bile salt on the pancreas and other vital organs such as the liver and the lung were investigated in rats. Intraductal injection (1 ml/kg) of α-chymotrypsin (50 - 100 mg/ml), trypsin (10 - 100 mg/ml), pancreatic elastase (10 mg/ml), lipase (100 - 300 mg/ml), pancreatic kallikrein (25 mg/ml) and sodium taurocholate (50 mg/ml) solutions significantly increased pancreatic water content. α-Chymotrypsin, pancreatic elastase, taurocholate and trypsin elicited gross pancreatic hemorrhage. In contrast, lipase and kallikrein elicited gross pancreatic edema, but not hemorrhage. Intravenous infusion of trypsin (1 mg/kg/hr) and pancreatic elastase (10 mg/kg/hr) significantly increased pulmonary vascular permeability in rats, whereas infusion of neutrophil elastase (0.3 mg/kg/hr) did not elicit these effects. Only trypsin slightly reduced arterial oxygen pressure. These results show pancreatic enzymes and bile salts induce pancreatic inflammation after retrograde injection into the pancreatic duct in rats. Furthermore, trypsin and pancreatic elastase extravasation into the vascular system can lead to pulmonary dysfunction in rats.

【 授权许可】

Unknown