Improved Method for Immobilization of Trypsin to Enhance Catalytic Performance of Trypsin in Confined Nanospaces

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Cheng Zhou¹  Tao Cen¹  Bo Jiang¹  Shubao Shen¹  Shemin Zhu²  Xiuming Wu¹ 

[1] College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology;College of Materials Science and Engineering, Nanjing University of Technology

关键词: Trypsin;    Immobilization;    Mesocellular Siliceous Foams;    Macromolecular Crowding;   

DOI  :  10.1252/jcej.43.595

来源: Maruzen Company Ltd

【 摘 要 】

References(29)Cited-By(3)Mesocellular siliceous foams (MCFs) functionalized with different groups were used in the trypsin immobilization process. Macromolecular reagents were co-assembled with trypsin in the MCFs to create a microenvironment resembling that in living cells. The catalytic characteristics and stability of trypsin preparations were investigated. The relative activity of immobilized trypsin was 122.9% when the enzyme was immobilized adsorptively in the MCFs. Trypsin assembled with BSA in the MCFs attained maximum specific activity, 1.33 times that of the unmodified immobilized trypsin. The Km value of the BSA derivative of trypsin was reduced to 15.9% and 16.6% relative to the valve for native and solely immobilized trypsin, respectively, while the Kcat/Km value of trypsin assembled with BSA was enhanced to 148%. The residual activity of trypsin assembled with BSA was 48.5% and 29.4% at 50°C and 60°C, respectively, after incubation for 60 min.

【 授权许可】

Unknown