Effect of Growth Factor on GTPase-Activating Protein (Ras GAP) in Chinese Hamster Ovary Cells

The Japanese Journal of Pharmacology

Yasuhiro Watanabe²  Shinya Arimura²  Tadashi Umekage²  Hiroko Nakata²  Misako Namima²  Mitsunao Kobayashi¹  Hidenori Sasa¹  Koji Tomiyama² 

[1] Department of Perinatal and Maternal Medicine, National Defense Medical College;Department of Pharmacology, National Defense Medical College

关键词: GTPase-activating protein;    Growth factor;    Immunoblot analysis;   

DOI  :  10.1254/jjp.76.121

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)GTPase-activating proteins (GAPs) stimulate the hydrolysis of GTP bound to small G-proteins and regulate the signal transduction pathway. Changes in the expression of p21-Ras p120-GAP induced by growth factor treatment were examined in cultured Chinese hamster ovary (CHO) and human choriocarcinoma (BeWo) cells. Expression of p120-GAP and GAP activity were measured. Fetal bovine serum induced a significant increased level of GAP in CHO cells, but did not increase GAP in BeWo cells. The results suggest that growth factors affect Ras GAP expression in CHO cells, while they do not in other cells such as BeWo cells.

【 授权许可】

Unknown