Effect of Human Neutrophil Elastase on Tracheal Mucociliary Transport in Anesthetized Quails

The Japanese Journal of Pharmacology

Hirofumi Kai²  Kazuo Takahama¹  Shusheng Tai²  Yoichiro Isohama²  Takeshi Miyata²  Tomoyuki Kido² 

[1] Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto University;Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University

关键词: Tracheal mucociliary transport;    Tracheal secretion;    Human neutrophil elastase;   

DOI  :  10.1254/jjp.75.439

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(5)We investigated the effect of human neutrophil elastase (HNE) on tracheal mucociliary transport in anesthetized quails. Topical application of HNE (30-300 μg/kg) to tracheal mucosa dosedependently decreased mucociliary transport velocity (MCTV). The HNE (300 μg/kg)-induced decrease in MCTV was blocked by ONO-5046·Na (sodium N-[2-[4-(2, 2-dimethylpropionyloxy)phenyl-sulfonylamino]benzoyl]aminoacetate tetrahydrate) (3-30 mg/kg, i.m.), a specific neutrophil elastase inhibitor. Furthermore, we found that HNE increased DNA, fucose and protein contents of tracheal lavages, and the increases were also reverted by ONO-5046·Na. These results indicated that HNE decreased tracheal mucociliary transport, and the decrease may be, at least in part, ascribed to the deterioration of tracheal secretions.

【 授权许可】

Unknown