New Curcuminoids Isolated from Zingiber cassumunar Protect Cells Suffering from Oxidative Stress: A Flow-Cytometric Study Using Rat Thymocytes and H202

The Japanese Journal of Pharmacology

Mami Nakata¹  Takayuki Nagano¹  Noriko Kajita¹  Lumi Chikahisa¹  Eisuke Okazaki¹  Toshiya Masuda¹  Yasuo Oyama¹ 

[1] Laboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima

关键词: Curcumin;    Curcuminoid;    Zingiber cassumunar;    Flow cytometry;    Oxidative stress;   

DOI  :  10.1254/jjp.75.363

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(25)Cited-By(17)Effects of new complex curcuminoids (cassumunin A and cassumunin B) isolated from tropical ginger, Zingiber cassumunar, were examined in dissociated rat thymocytes suffering from oxidative stress induced by 3 mM hydrogen peroxide by using a flow cytometer and ethidium bromide. The effects were compared with those of curcumin, a natural antioxidant, whose chemical structure is included in those of cassumunins A and B. Pretreatment of rat thymocytes with the respective cassumunins at concentrations ranging from 100 nM to 3 μM dose-dependently prevented the hydrogen peroxide (H202)-induced decrease in cell viability. It had the same action, although less effective, against the treatment with cassumunin A or B (3 μM) immediately after or 60 min after start of the oxidative stress. Respective potencies of cassumunins A and B in protecting the cells suffering from H202-induced oxidative stress were greater than that of curcumin. It is suggested that cassumunins A and B may possess a potent protective action on living cells suffering from oxidative stress.

【 授权许可】

Unknown