Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs

The Japanese Journal of Pharmacology

Masahiro Yamada¹  Takeshi Nabe¹  Shigekatsu Kohno¹  Yuji Saeki¹  Hideki Yamamura¹  Tomohiko Sekioka¹  Naoki Shinoda¹ 

[1] Department of Pharmacology, Kyoto Pharmaceutical University

关键词: Late asthmatic response;    Early asthmatic response;    Asthma;    Aluminum hydroxide;    IgE;   

DOI  :  10.1254/jjp.75.65

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(35)Cited-By(16)To develop a model of chronic experimental asthma in guinea pigs, the animal was forced to inhale the mist of a low dose of ovalbumin (OA) adsorbed on fine Al (OH) 3 for sensitization once every 4 weeks. The animal was challenged by inhalation with the mist of OA on day 14 after the respective sensitizations. Either the first or the second antigen challenge markedly induced an early asthmatic response (EAR), whereas there was hardly any late asthmatic response (LAR). At the 3rd challenge, LAR also emerged with some severity. These dual responses were consistently observed until the 10th challenge. On the other hand, repeated inhalation/challenge, once every 2 weeks, with OA alone at the same dose tended to lead to the desensitization of the EAR. In addition, LAR was hardly observed throughout the experiments. In both groups, γ1 and IgE levels in the serum were elevated by the repetitive antigen inhalations, yet no obvious relationship between these antibody levels and the intensity of either EAR or LAR was recognized. The present results indicate that the asthmatic model with reproducible EAR and LAR developed in this study appears to be very beneficial for the investigation of bronchial asthma and for the assessment of anti-asthma drugs.

【 授权许可】

Unknown