Antagonistic Effect of N-(3-Aminopropyl)cyclohexylamine on Neurotrophic Action of Spermine in Primary Cultured Rat Hippocampal and Cerebellar Neurons

The Japanese Journal of Pharmacology

Keijiro Samejima²  Kazuho Abe¹  Akira Shirahata²  Hiroshi Saito¹  Peng-Jiang Chu¹ 

[1] Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, The University of Tokyo;Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University

关键词: Spermine;    N-(3-Aminopropyl)cyclohexylamine;    Neuronal survival;    Regeneration;    Primary cultured neuron;   

DOI  :  10.1254/jjp.69.311

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(13)Cited-By(3)We previously found that spermine potently promotes the neuronal survival and regeneration of primary cultured brain neurons. N-(3-Aminopropyl)cyclohexylamine (APCHA) was originally developed as a spermine synthase inhibitor. To test if endogenous spermine biosynthesis contributes to neuronal survival and morphogenesis, we examined the effects of APCHA in primary cultured rat hippocampal and cerebellar neurons. APCHA at concentrations up to 10-6 M did not affect the neuronal survival, but significantly blocked the survival-promoting effect of spermine (10-8 M). APCHA also blocked the spermine-induced promotion of neurite regeneration following axotomy. Unlike APCHA, another cyclohexylamine derivative trans-4-methylcyclohexylamine did not affect the neurotrophic effect of spermine. These results suggest that in primary cultured brain neurons, APCHA works as a spermine antagonist rather than as a spermine synthesis inhibitor.

【 授权许可】

Unknown