The Japanese Journal of Pharmacology | |
Alteration of Liver Glutathione S-Transferase and Protease Activities by Cobalt Chloride Treatment of Rats | |
Yoko Aniya1  Ai Daido1  | |
[1] Laboratory of Physiology and Pharmacology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus | |
关键词: Cobalt chloride; Glutathione S-transferase; Liver microsome; Enzyme induction; Protease; | |
DOI : 10.1254/jjp.66.357 | |
学科分类:药理学 | |
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society | |
![]() |
【 摘 要 】
References(26)Cited-By(2)Effects of cobalt chloride on liver glutathione S-transferase and protease activities were studied. When cobalt chloride (60 mg/kg) was given to rats, liver microsomal glutathione S-transferase and protease activities were significantly increased 24 hr after the injection, whereas glutathione peroxidase activity in microsomes was decreased. The increase in glutathione S-transferase activity caused by cobalt chloride treatment was not reversed by dithiothreitol, and the activation of the transferase by N-ethylmaleimide was similar to that of the control, indicating that the increase in the transferase activity by cobalt chloride is not due to a modification of the sulfhydryl group of the enzyme. Immunochemical analysis of the liver microsomes did not detect any proteolytic product of microsomal glutathione S-transferase. In puromycinor actinomycin D-treated rats, an increase in the transferase activity caused by cobalt chloride treatment was depressed. Thus it was suggested that liver microsomal glutathione S-transferase is induced by cobalt chloride treatment, but not activated by limited proteolysis via microsomal protease.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912080713773ZK.pdf | 428KB | ![]() |