Alteration of Liver Glutathione S-Transferase and Protease Activities by Cobalt Chloride Treatment of Rats

The Japanese Journal of Pharmacology

Yoko Aniya¹  Ai Daido¹ 

[1] Laboratory of Physiology and Pharmacology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus

关键词: Cobalt chloride;    Glutathione S-transferase;    Liver microsome;    Enzyme induction;    Protease;   

DOI  :  10.1254/jjp.66.357

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(26)Cited-By(2)Effects of cobalt chloride on liver glutathione S-transferase and protease activities were studied. When cobalt chloride (60 mg/kg) was given to rats, liver microsomal glutathione S-transferase and protease activities were significantly increased 24 hr after the injection, whereas glutathione peroxidase activity in microsomes was decreased. The increase in glutathione S-transferase activity caused by cobalt chloride treatment was not reversed by dithiothreitol, and the activation of the transferase by N-ethylmaleimide was similar to that of the control, indicating that the increase in the transferase activity by cobalt chloride is not due to a modification of the sulfhydryl group of the enzyme. Immunochemical analysis of the liver microsomes did not detect any proteolytic product of microsomal glutathione S-transferase. In puromycinor actinomycin D-treated rats, an increase in the transferase activity caused by cobalt chloride treatment was depressed. Thus it was suggested that liver microsomal glutathione S-transferase is induced by cobalt chloride treatment, but not activated by limited proteolysis via microsomal protease.

【 授权许可】

Unknown