Microsomal Ca2+‐ and phospholipid‐dependent protein kinase

FEBS Letters

Sommarin, Marianne¹  Jergil, Bengt¹ 

[1] Biochemistry, Chemical Centre, POB 740, S-220 07 Lund, Sweden

关键词: Ca2+;    Liver microsome;    Membrane binding;    Phospholipid;    Protein kinase;   

DOI  :  10.1016/0014-5793(83)80502-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Ca²⁺- and phospholipid-dependent protein kinase (CaPK) has been identified in rat liver microsomes. CaPK isolated from liver cytosol bound to smooth microsomes in the presence of 100 μM CaCl₂. A saturation in binding was observed when a 5-fold excess of enzyme over that present in microsomes had become bound. The microsomal CaPK and 50% of the enzyme bound in vitro was not removed by EGTA treatment. This suggests that Ca²⁺ is required for the binding of CaPK to microsomes, but not for the retention of the enzyme on the membrane.

【 授权许可】

Unknown