Cobra polypeptide cytotoxin I and marine worm polypeptide cytotoxin A‐IV are potent and selective inhibitors of phospholipid‐sensitive Ca2+‐dependent protein kinase

FEBS Letters

Kuo, J.F.¹  Schatzman, Randall C.¹  Turner, R.Scott¹  Raynor, Robert L.¹  Kem, William R.¹  Mazzei, Gonzalo J.¹ 

[1] Department of Pharmacology, Emory University School of Medicine, Atlanta, GA 30322 USA

关键词: Ca2+;    Phospholipid;    Protein phosphorylation;    Neurotoxin;    PL-Ca-PK;    phospholipid-sensitive Ca2+-dependent protein kinase;    MLCK;    myosin light chain kinase;    A-PK and G-PK;    cyclic AMP- and cyclic GMP-dependent protein kinases;    respectively;   

DOI  :  10.1016/0014-5793(83)80144-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of a number of polypeptide cytotoxins and neurotoxins on various protein kinases were examined. It was found that cobra cytotoxin I and marine worm cytotoxin A-IV effectively and specifically inhibited phospholipid-sensitive Ca²⁺-dependent protein kinase (PL-Ca-PK) relative to myosin light chain kinase and cyclic nucleotide-dependent protein kinases. Inhibition of PL-Ca-PK by these cytotoxins could be overcome by phosphatidylserine. Neurotoxins, in comparison, were much less effective inhibitors. The present findings indicated that these polypeptide cytotoxins, unlike other agents reported to date, were selective inhibitors of PL-Ca-PK and could be used to differentiate Ca²⁺-dependent events regulated by phospholipid or calmodulin.

【 授权许可】

Unknown