Effects of Kamikihito, a Traditional Chinese Medicine, on Neurotransmitter Receptor Binding in the Aged Rat Brain Determined by In Vitro Autoradiography (2): Changes in GABAA and Benzodiazepine Receptor Binding

The Japanese Journal of Pharmacology

Takao Kaneyuki²  Tadaomi Morimasa²  Kiyofumi Yamada¹  Takaaki Hasegawa¹  Tsutomu Kameyama³  Toshikiyo Shohmori²  Seiichi Ishihara³  Tetsuo Hayashi¹  Toshitaka Nabeshima¹ 

[1] Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University School of Medicine;Department of Neurology, Okayama University Medical School;Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Meijo University

关键词: Kamikihito;    Aged rat;    [3H]Muscimol binding;    [3H]Flunitrazepam binding;    Autoradiography;   

DOI  :  10.1254/jjp.66.53

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(20)Cited-By(6)We investigated the effects of the long-term administration of Kamikihito (KKT) on the specific binding of [3H]muscimol and [3H]flunitrazepam in the brains of young and aged rats using in vitro quantitative autoradiography. Specific [3H]muscimol binding in aged rats was decreased in all brain regions examined compared with that in young rats, whereas [3H]flunitrazepam binding did not change in any of the brain regions. Scatchard analysis revealed that the maximal number of [3H]muscimol binding sites in the cortex and thalamus was significantly decreased in aged rats compared with young rats, while its affinity remained unchanged. Long-term administration of KKT in young rats had no effect on either [3H]muscimol or [3H]-flunitrazepam binding. In contrast, the same treatment in aged rats produced a significant increase in [3H]-flunitrazepam binding to the cortex, caudate/putamen and accumbens, and it tended to decrease the [3H]-muscimol binding. These results suggest that the selective reduction of specific [3H]muscimol binding in the brain may be responsible, at least in part, for anxiety-related behavior in aged rats. Furthermore, it appears that the significant increase in specific [3H]flunitrazepam binding produced in the brains of aged rats by the long-term administration of KKT may be responsible for the anxiolytic effects of this agent.

【 授权许可】

Unknown