Human Aortic Smooth Muscle Cells Containing Angiotensin II Type 1 Receptors

The Japanese Journal of Pharmacology

Hidenori Yazawa¹  Kazuo Honda¹  Emi Iida-Kubota¹ 

[1] Drug Serendipity Research Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd.

关键词: Angiotensin II;    Receptor;    Aorta (human);    Smooth muscle cell;   

DOI  :  10.1254/jjp.62.339

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(20)Cited-By(5)We characterized the angiotensin II (All) receptor in human aortic smooth muscle cells (HASMCs). This receptor binds [125I]Sar1, Ile8-angiotensin II with a high affinity of 0.20±0.04 nM and a low capacity of 5.3±0.4 fmol/mg protein (230±17 sites/cell). Based on the K1 values, the ranking order of [125I]Sar1, Ile8-AII binding inhibition was as follows: Sar1, Ile8AII > AII > Dup 753 > All > AI » PD 123319. The addition of AII to HASMCs induced a rapid, transient increase in intracellular free Ca2+ concentration followed by a lower, sustained phase. When extracellular Ca2+ was removed by adding 3 mM EGTA, this initial transient increase was not changed, but the sustained phase was abolished. These results revealed AII receptors in HASMCs to be of the type 1 receptor subtype, which induce Ca2+ mobilization mainly from intracellular Ca2+ stores.

【 授权许可】

Unknown