Isolation and Characterization of Sweat Gland Myoepithelial Cells from Human Skin

Cell Structure and Function

Hiroyuki Murota²  Ichiro Katayama²  Kiyotoshi Sekiguchi¹  Ryuichiro Kurata¹  Itsuko Nakano¹  Atsushi Tanemura²  Sugiko Futaki¹ 

[1] Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University;Department of Dermatology, Graduate School of Medicine, Osaka University

关键词: Skin;    epidermis;    mammary glands;    multipotency;    self-renewal ability;   

DOI  :  10.1247/csf.14009

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(30)Cited-By(2)Supplementary materials(2)Stem cells routinely maintain the main epidermal components, i.e. the interfollicular epidermis, hair follicles, and sweat glands. Human sweat glands present throughout the body are glandular exocrine organs that mainly play a role in thermoregulation by sweating. Emerging evidence points to the presence of stem cells in sweat glands, but it remains unclear whether such stem cells exist in human sweat glands. Here, we attempted to gather evidence for stem cells in human sweat glands, which would be characterized by self-renewal ability and multipotency. First, we explored human sweat gland cells for expression of stem cell markers. CD29 and Notch, epidermal stem cell markers, were found to reside among α-smooth muscle actin-positive myoepithelial cells in human sweat glands. Next, sweat gland myoepithelial cells were isolated from human skin as a CD29hiCD49f hi subpopulation. The myoepithelial cell-enriched CD29hiCD49f hi subpopulation possessed the ability to differentiate into sweat gland luminal cells in sphere-forming assays. Furthermore, CD29hiCD49f hi subpopulation-derived sphere-forming cells exhibited long-term proliferative potential upon multiple passaging, indicating that the CD29hiCD49f hi myoepithelial subpopulation includes stem cells with self-renewal ability. These findings provide evidence that human sweat gland myoepithelial cells contain stem cells that possess both self-renewal ability and multipotency to differentiate into sweat glands.

【 授权许可】

Unknown