Discovery of a stem-like multipotent cell fate

American Journal of Stem Cells

Emily S Paffhausen¹  Yasir Alowais² 

[1] Department of Biology, American University, 4400 Massachusetts Ave NW, Washington DC 20016, USA;Georgetown Lombardi Comprehensive Cancer Center, 3800 Reservoir Road NW, Washington DC 20057, USA

关键词: Adipose-derived stem cells;    multipotency;    adipocyte;    chondrocyte;    osteoblast;   

DOI  :  

学科分类：分子生物学，细胞生物学和基因

来源: e-Century Publishing Corporation

【 摘 要 】

Adipose derived stem cells (ASCs) can be obtained from lipoaspirates and induced in vitro to differentiate into bone, cartilage, and fat. Using this powerful model system we show that after in vitro adipose differentiation a population of cells retain stem-like qualities including multipotency. They are lipid (-), retain the ability to propagate, express two known stem cell markers, and maintain the capacity for trilineage differentiation into chondrocytes, adipocytes, and osteoblasts. However, these cells are not traditional stem cells because gene expression analysis showed an overall expression profile similar to that of adipocytes. In addition to broadening our understanding of cellular multipotency, our work may be particularly relevant to obesity-associated metabolic disorders. The adipose expandability hypothesis proposes that inability to differentiate new adipocytes is a primary cause of metabolic syndrome in obesity, including diabetes and cardiovascular disease. Here we have defined a differentiation-resistant stem-like multipotent cell population that may be involved in regulation of adipose expandability in vivo and may therefore play key roles in the comorbidities of obesity.

【 授权许可】

CC BY-NC