2D-DIGE Proteomic Analysis of Mesenchymal Stem Cell Cultured on the Elasticity-tunable Hydrogels

Cell Structure and Function

Thasaneeya Kuboki¹  Fahsai Kantawong³  Matthew J Dalby³  Richard Burchmore²  Satoru Kidoaki¹ 

[1] Institute for Materials Chemistry and Engineering, Division of Biomolecular Chemistry, Kyushu University;Sir Henry Welcome Functional Genomics Facility, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow;Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow

关键词: Mesenchymal stem cell;    surface elasticity;    2D-DIGE;    cytoskeletal proteins;    cellular tensegrity;   

DOI  :  10.1247/csf.12012

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(68)Supplementary materials(10)The present study focuses on mechanotransduction in mesenchymal stem cells (MSCs) in response to matrix elasticity. By using photocurable gelatinous gels with tunable stiffness, proteomic profiles of MSCs cultured on tissue culture plastic, soft (3 kPa) and stiff (52 kPa) matrices were deciphered using 2-dimensional differential in-gel analysis (2D-DIGE). The DIGE data, tied to immunofluorescence, indicated abundance and organization changes in the cytoskeletonal proteins as well as differential regulation of important signaling-related proteins, stress-responsing proteins and also proteins involved in collagen synthesis. The major CSK proteins including actin, tubulin and vimentin of the cells cultured on the gels were remarkably changed their expressions. Significant down-regulation of α-tubulin and β-actin can be observed on gel samples in comparison to the rigid tissue culture plates. The expression abundance of vimentin appeared to be highest in the MSCs cultured on hard gels. These results suggested that the substrate stiffness significantly affects expression balances in cytoskeletal proteins of MSCs with some implications to cellular tensegrity.

【 授权许可】

Unknown