Magnetic Separation of Human Podocalyxin-like Protein 1 (hPCLP1)-Positive Cells from Peripheral Blood and Umbilical Cord Blood Using Anti-hPCLP1 Monoclonal Antibody and Protein A Expressed on Bacterial Magnetic Particles

Cell Structure and Function

Tomoko Yoshino¹  Haruko Takeyama¹  Miho Shiokawa³  Akihiko Yabuhara⁴  Motoki Kuhara³  Tadashi Matsunaga¹  Tomoya Okabe²  Shinji Mizoguchi³ 

[1] Department of Biotechnology, Tokyo University of Agriculture and Technology;ACTGen. Inc.;Technology and Development Division, Medical & Biological Laboratories Co. Ltd.;Pediatrics Department, Ina Central Hospital

关键词: hematopoietic progenitor cells;    podocalyxin-like protein 1 (PCLP1);    bacterial magnetic particles (BacMPs);    cell separation;   

DOI  :  10.1247/csf.08043

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(28)Cited-By(4)Hemangioblasts are common progenitors of hematopoietic and angiogenic cells, which have been demonstrated in the mouse to possess a unique cell surface marker, podocalyxin-like protein 1 (PCLP1) (Hara, T. et al., Immunity, 11: 567–578. 1999). In this study, we prepared a novel monoclonal antibody against human PCLP1 (hPCLP1) and attempted to isolate human hematopoietic progenitor cells from umbilical cord blood and peripheral blood using nano-sized bacterial magnetic particles (BacMPs) coupled with the anti-hPCLP1 antibody. Flow cytometric analysis demonstrated that the purity of separated hPCLP1-positive cells from peripheral blood was approximately 95% whereas peripheral blood mononuclear cells contained only 0.1% PCLP1+ cells. Umbilical cord blood was demonstrated to be a better source for PCLP1+ cells than peripheral blood. These results suggest that the separation of human PCLP1+ cells using BacMPs with anti-hPCLP1 were extremely effective and may be useful as a means to prepare human hematopoietic progenitor cells.

【 授权许可】

Unknown