Identification of Jmjd1a as a STAT3 Downstream Gene in mES Cells

Cell Structure and Function

Sang Yeul Han¹  Song Yi Ko¹  Hye Yoon Kang¹  Hyun Sil Lee¹  Seung Hwan Hong¹ 

[1] School of Biological Sciences, and Institute of Molecular Biology and Genetics, Seoul National University

关键词: embryonic stem cells;    LIF;    STAT3;    DD-RT PCR;    differentiation;   

DOI  :  10.1247/csf.31.53

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(34)Cited-By(10)Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. To look for STAT3-downstream target genes, we performed DD-RT PCR in the presence or absence of LIF. Through further confirmation, we finally selected 8 genes whose expressions were significantly dependent upon the presence of LIF. Among them, Jmjd1a was down-regulated after LIF withdrawal, and it was selected for further investigation. Its expression started to decrease 1 day after the removal of LIF, and disappeared on day 3. It was also shown that STAT3 could bind to the promoter region of Jmjd1a gene. These data demonstrate that Jmjd1a might be a critical signaling molecule underlying the maintenance of pluripotency in mES cells.

【 授权许可】

Unknown