Utilization of Liposomes in Vesicular Transport Studies

Cell Structure and Function

Masahiro Kinuta¹  Kohji Takei¹ 

[1] Department of Neuroscience, Okayama University Graduate School of Medicine and Dentistry

关键词: liposome;    vesicular transport;    coat proteins;    phospholipids;    light scattering;    endocytosis;   

DOI  :  10.1247/csf.27.63

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(49)Cited-By(8)Various coated vesicles are implicated in the intracellular transport between different compartments. In vitro reconstitution is a powerful experimental system to study molecular mechanisms involved in assembly of coat proteins from cytosol onto membranes as well as formation of coated vesicles. Liposomes have been recently utilized in the cell-free systems. In this review, we summarize studies on reconstitutions of coated vesicles or coated structures on liposomes. A novel method using dynamic light scattering (DLS) to quantify vesicle formation from liposomes also is described. Our recent study on the role of phospholipids in vesicle formation, where the DSL assay is used in combination with lipid analysis, also is introduced.

【 授权许可】

Unknown