Insulin-Degrading Enzyme Exists Inside of Rat Liver Peroxisomes and Degrades Oxidized Proteins

Cell Structure and Function

Masashi Morita⁴  Sadaki Yokota⁵  Ryuichiro Sato¹  Kiyoto Motojima²  Sataro Goto²  Shoji Okamura⁴  Tatsuya Takano³  Tsuneo Imanaka⁴  Igor V Kurochkin² 

[1]Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, The University of Tokyo

[2]Department of Biochemistry, School of Pharmaceutical Sciences, Toho University

[3]Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University

[4]Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

[5]Biological Laboratory, Yamanashi Medical University

关键词: insulin-degrading enzyme;    subcellular localization;    peroxisomes;    proteolysis;    oxidized proteins;   

DOI  :  10.1247/csf.25.309

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(32)Cited-By(22)Insulin-degrading enzyme (IDE) was detected by immunoblot analysis in highly purified rat liver peroxisomes. IDE in the peroxisomal fraction was resistant to proteolysis by trypsin and chymotrypsin under conditions where the peroxisomal membranes remained intact. After sonication of the peroxisomal fraction, IDE was recovered in the supernatant fraction. Further, the localization of IDE in the peroxisomes was shown by immunoelectron microscopy. In addition, IDE isolated from peroxisomes degraded insulin as well as oxidized lysozyme as a model substrate for oxidized proteins. These results suggest that IDE exists in an active form in the matrix of rat liver peroxisomes and is involved in elimination of oxidized proteins in peroxisomes.

【 授权许可】

Unknown