Expression of Neural Cell Adhesion Molecule LI in Human Lung Cancer Cell Lines

Cell Structure and Function

Hiromi Wada¹  Akihiko Iwamatsu²  Tohru Masuda⁴  Kazuhiro Ishii³  Keizo Furuke³  Masahiro Katayama³  Hiroshi Masutani³  Kazumasa Takeda³ 

[1] Chest Disease Research Institute, Kyoto University;Central Laboratories for Key Technology;Institutefor Virus Research, Kyoto University;Department of Immunobiology, Faculty of Medicine, Kyoto University

关键词: cell adhesion molecule L1;    carcinoma;    lung cancer;    neuroblastoma;    monoclonal antibody;   

DOI  :  10.1247/csf.22.511

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(33)Cited-By(7)To investigate neuron-specific antigens, hybridoma cells were produced between mouse spleen cells immunized with human neuroblastoma cells (IMR-32) and mouse myeloma cells. 247 hybridoma clones were harvested and one of them was further cultured for recloning. Eventually, one hybridoma clone was obtained and its antibody was designated N-A8. The characteristics of this antibody were determined by immunostaining and flow cytometry. First, the antibody recognized the surface antigens of IMR-32 cells. Second, unexpectedly, N-A8 was reactive not only with human neuroblastoma cell lines but also with human lung cancer cell lines. As analyzed by immunoprecipitation method and SDS-PAGE, the molecular size of the antigen recognized by N-A8 was 210 kDa. The antigen was then purified by affinity chromatography and identified as neural adhesion molecule LI by amino acid sequence analysis. By the present investigation, it was clearly demonstrated that LI is expressed in human lung cancer cells.

【 授权许可】

Unknown