Association of hnRNP SI Proteins C2 and D2 with Vimentin Intermediate Filaments

Cell Structure and Function

Akira Inoue³  Katsuji Tsugawa⁴  Angela Mai¹  Daisaburo Fujimoto¹  Takanori Watanabe³  Kenichi P. Takahashi² 

[1] Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology;Department of Anatomy and Physiology, Osaka Prefectural College of Health Sciences;Department of Biochemistry, Osaka City University Medical School;Department of Natural Science, Osaka Women's University

关键词: SI protein;    hnRNP;    intermediate filament;    microtubule;    microfilament;    vimentin;   

DOI  :  10.1247/csf.22.239

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(15)Cited-By(3)SI proteins A-D are hnRNP proteins which were originally isolated from cell nuclei of various tissues, by selective extraction at pH 4.9 from the supernatants of nuclei mildly treated with DNase I or RNase A. In the present study, a hybridoma was isolated which produced a monoclonal antibody that reacted specifically with SI proteins C2 and D2. When the antibody was used in indirect immunofluorescence staining of cultured cells, it stained, in addition to the nuclei, the cytoskeleton-like fibrous structures in the cytoplasm. We demonstrate that the cytoskeletal filaments are vimentin intermediate filaments. This is the first report on the hnRNP protein-association with cytoskeleton, and will help to clarify cytoplasmic mRNA localization as well as cytoplasmic distribution of hnRNP proteins.

【 授权许可】

Unknown