Induction of mcll/EAT, Bcl-2 Related Gene, by Retinoic Acid or Heat Shock in the Human Embryonal Carcinoma Cells, NCR-G3

Cell Structure and Function

Akihiro Umezawa¹  Johji Inazawa³  Tatsuya Maruyama¹  Shin-ichiro Imai²  Toshiya Takano²  Jun-ichi Hata¹ 

[1] Department of Pathology, Keio University School of Medicine;Department of Microbiology, Keio University School of Medicine;Department of Hygiene, Kyoto Prefectural University of Medicine

关键词: embryonal carcinoma cells;    mcl1;    retinoic acid;    heat shock;    differentiation;   

DOI  :  10.1247/csf.21.143

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(32)Cited-By(11)NCR-G3 cells were established from a testicular embryonal carcinoma and were differentiated into multi-lineages including trophectoderm cells by exposure to retinoic acid. The differentiated cells began to produce human chorionic gonadotropin (hCG), a trophectoderm-specific hormone, which was regulated at the mRNA level. As we assumed that genes responsible for differentiation were differentially expressed at the early stage of retinoic acid-induced differentiation, we prepared a cDNA library from retinoic acid-treated NCR-G3 cells. This cDNA library was then screened for genes whose expression was induced during the differentiation of these cells. From about 5 × 104 clones screened, three independent sequences were isolated. Sequencing analysis revealed that clone 1002 codes for mcll/EAT, which has a Bcl-2 homology domain. The expression of mcll/EAT, the Bcl-2 related gene, was increased at an early stage of the retinoic acid-induced differentiation and preceded the up-regulation of cytokeratin and hCG genes after ratinoic acid treatment. Furthermore, mcll/EAT was also up-regulated by heat shock, which has recently been shown to induce the cells to differentiate.

【 授权许可】

Unknown