Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress

Cells

Ibtissam Echchgadda¹  Caleb C. Roth²  Cesario Z. Cerna³ 

[1] Air Force Research Laboratory, 711th Human Performance Wing, Human Effectiveness Directorate, Bioeffects Division, Radio Frequency Bioeffects Branch, 4141 Petroleum Road, Bldg. 3260, Fort Sam Houston, TX 78234, USA; E-Mail:;Department of Radiology, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, USA; E-Mail:;General Dynamics Information Technology, Fort Sam Houston, TX 78234, USA; E-Mail:

关键词: keratinocytes;    heat shock;    gene expression;    cellular stress response;    bioinformatics;   

DOI  :  10.3390/cells2020224

来源: mdpi

【 摘 要 】

The gene expression kinetics for human cells exposed to hyperthermic stress are not well characterized. In this study, we identified and characterized the genes that are differentially expressed in human epidermal keratinocyte (HEK) cells exposed to hyperthermic stress. In order to obtain temporal gene expression kinetics, we exposed HEK cells to a heat stress protocol (44 °C for 40 min) and used messenger RNA (mRNA) microarrays at 0 h, 4 h and 24 h post-exposure. Bioinformatics software was employed to characterize the chief biological processes and canonical pathways associated with these heat stress genes. The data shows that the genes encoding for heat shock proteins (HSPs) that function to prevent further protein denaturation and aggregation, such as HSP40, HSP70 and HSP105, exhibit maximal expression immediately after exposure to hyperthermic stress. In contrast, the smaller HSPs, such as HSP10 and HSP27, which function in mitochondrial protein biogenesis and cellular adaptation, exhibit maximal expression during the “recovery phase”, roughly 24 h post-exposure. These data suggest that the temporal expression kinetics for each particular HSP appears to correlate with the cellular function that is required at each time point. In summary, these data provide additional insight regarding the expression kinetics of genes that are triggered in HEK cells exposed to hyperthermic stress.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.