cDNA Cloning and Sequence Analysis of a Novel Calcium Binding Protein with Oligoproline Motif

Cell Structure and Function

Takashi Muramatsu¹  Hisako Muramatsu¹  Teruo Miyauchi²  Masayuki Ozawa¹  Su Yu¹  Apala Farhat Naved¹ 

[1] Department of Biochemistry, Faculty of Medicine, Kagoshima University;Japan Immunoresearch Laboratories

关键词: Calcium binding protein;    lectin binding site;    oligoproline;    Src homology region III;   

DOI  :  10.1247/csf.20.263

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(16)Cited-By(4)Using antibodies against pokeweed agglutinin-binding proteins from F9 embryonal carcinoma cells, we isolated a cDNA clone PW29 reacting with the antibody. MRNA hybridizing with the cDNA was 4.7 kb, and was strongly expressed in the testis, brain, kidney and heart as well as in F9 cells. The size of mRNA in the testis was heterogeneous. Sequencing the cDNA clone revealed a putative polypeptide of 70 kDa, which is rich in hydrophilic amino acids and has a characteristic sequence of (GluLys)5. The protein also has an oligoproline motif, which conforms to the rule of binding capability to Src homology region III. The cDNA was translated as a fusion protein with glutathione-S-transferase, and was verified to have calcium binding activity, upon a calcium blot experiment.

【 授权许可】

Unknown