Immunocytochemical Study of Microvilli in a Gastric Carcinoma-derived Cell Line

Cell Structure and Function

Hidehiko Ohta³  Tadayoshi Uezato¹  Isamu Kino²  Yasuhisa Naito²  Yi-Xin Wu¹  Michiya Fujita¹  Masako Kasami² 

[1] Department of Biochemistry, Hamamatsu University, School of Medicine;Department of Pathology, Hamamatsu University, School of Medicine;Kyorin University

关键词: Microvilli;    villin;    ezrin;    KATO-III cell;   

DOI  :  10.1247/csf.20.245

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(22)To study the structural components of microvilli of the KATO-III cell, we used anti-villin, -ezrin, and anti-MVM (microvillous membrane prepared against mouse intestinal microvilli) antibodies. Villin and ezrin cross-link actin bundles of microvilli such as those in the small intestine and renal proximal cells. Electromicroscopically, the cytoskeletal core of microvilli of the KATO-III cell was constituted of actin-filament bundles. The anti-villin antibody but not anti-ezrin antibody reacted with the KATO-III cell as demonstrated by FITC-immunofluorescence and PAP-staining. Anti-villin, anti-MVM, but not anti-ezrin antibody, reacted with the KATO-III cell surface and with intracellular materials. Western blot analysis using anti-villin and anti-MVM antibodies revealed proteins of -95 kDa (villin), and 15 kDa in the microsomal membrane fractions of KATO-III cells, respectively. Immunocytochemical and confocal laser microscopic studies showed that the cell-surface and the intracellular microcysts of KATO-III cells were preferentially decorated by anti-villin and anti-MVM antibodies. These data suggested that some actin-binding proteins, such as villin, were localized at the cell surface and on some of the intracellular cytoplasmic structures of the KATO-III cell.

【 授权许可】

Unknown