A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokine receptor gp130

FEBS Letters

Bates, Paul A.¹  Kerr, Ian M.¹  Lillemeier, Björn F.¹  Strobl, Birgit¹  Is'harc, Hayaatun¹  Behrmann, Iris²  Hilkens, Catharien M.U.¹ 

[1]Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK

[2]Biochemistry, RWTH Aachen, Pauwelstr. 30, 52057 Aachen, Germany

关键词: Jak kinase;    FERM domain;    gp130;    Cytokine receptor;    EpoR;    erythropoietin receptor;    FERM;    four-point-one;    ezrin;    radixin;    moesin;    IFN;    interferon;    IL;    interleukin;    Jak;    Janus kinase;    STAT;    signal transducer and activator of transcription;   

DOI  :  10.1016/S0014-5793(01)02783-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The terminal portion of the Janus kinases (Jaks) contains a divergent FERM (our-point-one, zrin, adixin, oesin) homology domain comprising 19 conserved hydrophobic regions. To determine the role of this domain in governing recruitment of Jak1, but not Jak3, to the gp130 subunit of the interleukin-6 family of cytokine receptors, the interaction of three Jak1/Jak3 chimeras with gp130 was investigated. Chimeras 1, 2 and 3 (Jak1 FERM regions 1–19, 1–18 and 1–8/Jak3, respectively) were all enzymically active. Chimeras 1 and 2 interacted with the cytoplasmic domain of gp130, although less efficiently than Jak1. Only chimera 2, however, restored gp130 signalling in Jak1-negative cells. The data are consistent with recruitment of Jak1 to gp130 through the Jak1 FERM domain, but also emphasise the likely requirement for precise Jak/receptor orientation to sustain function.

【 授权许可】

Unknown