Apoptosis by Demecolcine in V79 Cells

Cell Structure and Function

Zhi-ping Zong²  Kohzaburo Fujikawa-Yamamoto²  Shizuo Odashima¹  Kouhei Teraoka²  Hiroko Yamagishi² 

[1] Department of Pathology, Kanazawa Medical University;Division of Basic Science, Research Institute of Medical Science

关键词: apoptosis;    V79 cells;    demecolcine;    hyperploidization;   

DOI  :  10.1247/csf.19.391

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(12)Cited-By(2)Demecolcine (Colcemid), an inhibitor of spindle fiber formation in M phase, induced apoptosis in V79 cells. At a concentration of 0.01μg/ml demecolcine, V79 cells proliferated exponentially as well as controls, although temporal M phase accumulation occurred 6 h after the addition of demecolcine. At 0.1 μg/ml, the cells became hyperploid after remaining in the M phase for some time. Apoptosis occurred in V79 cells exposed to demecolcine at a concentration of 0.03μg/ml. Apoptosis was defined as the appearance of a sub-G1 peak in DNA histograms and a ladder pattern of fragmented DNA in gelelectrophoresis.

【 授权许可】

Unknown