Further Analysis of The Effect of Ca2+ on Morphogenesis of HVJ (Sendai Virus) in LLC-MK2 Cells : Effects on Phosphorylated M Protein Associated with Viral Morphogenesis

Cell Structure and Function

Masakazu Kamata¹  Jeman Kim¹ 

[1] Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University

关键词: HVJ (Sendai virus);    effect of Ca2+;    M protein;    HVJ morphogenesis;    phosphorylation;   

DOI  :  10.1247/csf.19.325

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(30)Cited-By(3)Previously we have reported that Ca2+ concentration in culture medium affects the morphogenesis of HVJ from infected cells. We further investigated the effects of Ca2+ on HVJ morphogenesis in LLC-MK2 cells, focusing on M (matrix) protein which plays an important role in the assembly of viral components. On culture in standard medium (containing 1.8 mM Ca2+), most of the M protein accumulated on the apical surface in patches. On double immunofluorescent staining with anti-M protein monoclonal antibody and polyclonal antibody against HVJ glycoproteins, M protein and viral glycoproteins were observed in the same patches at the cell surface. By contrast, in low Ca2+ medium, M protein was evenly dispersed over the cell surface, especially in the regions of cell-cell contact, and was not seen in patches. Immunoblot analysis for M protein in the infected cells showed that the total amount of M protein was almost the same irrespective of Ca2+ concentration in the culture medium. But, there was a distinct difference in the forms of M protein found in the cells : most of the M protein in the cells cultured in standard medium was of the non-phosphorylated form, whereas the phosphorylated form of M protein was distinctly increased in the cells cultured in low Ca2+ medium. These results suggest that Ca2+-deficiency in the medium blocks dephosphorylation of phosphorylated M protein necessary for patch formation, resulting in disturbance of viral morphogenesis.

【 授权许可】

Unknown