Suppression of Enveloped Virus Production with a Substance from Silkworm Faeces

Cell Structure and Function

Akihiro Hiraki²  Yoko Ikuno²  Jeman Kim²  Shigeharu Ueda¹ 

[1] Research Institute for Microbial Disease, Osaka University;Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University

关键词: viral production;    silkworm faeces;    HVJ (Sendai virus);    HVJ morphogenesis;    enveloped virus;   

DOI  :  10.1247/csf.21.501

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(23)Cited-By(5)In the course of investigating effective biological active substances, we detected a substance in an extract of silkworm faeces that markedly suppresses viral production. The extract, prepared with hot phosphate-buffered saline and purified with ammonium sulfate precipitation, inhibited HVJ (Sendai virus), HSV (herpes simplex virus type-1), and HIV (human immunodeficiency virus type-1), but not poliovirus, suggesting that it is effective on enveloped virus production but not on non-enveloped ones. In the case of HVJ, indirect immunofluorescent staining using anti-HVJ antibody and Northern blotting analysis showed that, while viral adsorption and entry into the host cells were not affected, the synthesis of viral specific gene was inhibited by pretreatment of the virions with the extract. The extract affected more effectively aged virion, which loses membrane function as barrier and its envelope is leaky, than young virion that maintains barrier function. The active substance was partially purified by gel filtration after treatment of the extract with 1 N NaOH solution. From analysis with SDS-PAGE (SDS-polyacrylamide gel electrophoresis), protein bands were detected with molecular masses of about 25 kDa and near 14 kDa, while sugars were also detected with lectin blotting.

【 授权许可】

Unknown