Microsomal Aldehyde Dehydrogenase or Its Cross-Reacting Protein Exists in Outer Mitochondrial Membranes and Peroxisomal Membranes in Rat Liver

Cell Structure and Function

Akitsugu Yamamoto²  Yutaka Tashiro²  Ryuichi Masaki²  Yukio Fujiki¹  Katsuji Miyauchi² 

[1] Laboratory of Molecular Cell Biology, Meiji Institute of Health Science;Department of Physiology and Liver Research Center (Cell Biology), Kansai Medical University

关键词: osteoblasts;    differentiation;    gap junctions;    connexins;    human;   

DOI  :  10.1247/csf.18.427

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(29)Cited-By(7)We investigated the subcellular distribution of microsomal aldehyde dehydrogenase (msALDH) in rat liver and revealed by the immunoblotting method that msALDH or a cross-reacting 54-kDa protein(s) exists in the outer mitochondrial membranes and peroxisomes. Anti-msALDH antibody markedly inhibited the decanal aldehyde dehydrogenase activity of the outer mitochondrial membranes as well as that of the microsomes. Immunogold electron microscopic observations showed that gold particles are localized over the ER, outer mitochondrial membranes and peroxisomal membranes. These results suggest that msALDH or its crossreacting related protein is distributed not only in the ER membranes but also in the mitochondrial outer membranes and peroxisomal membranes.

【 授权许可】

Unknown