Relationship between the Expression of the Gap Junction Protein and Osteoblast Phenotype in a Human Osteoblastic Cell Line during Cell Proliferation

Cell Structure and Function

Seiichi Ishii¹  Shintaro Nomura²  Hideki Chiba³  Michio Mori³  Masahito Oyamada³  Takashi Kojima³  Norimasa Sawada³ 

[1] Departments of Orthopedic Surgery, Sapporo Medical University School of Medicine;Department of Pathology, Osaka University Medical School;Departments of Pathology, Sapporo Medical University School of Medicine

关键词: osteoblasts;    differentiation;    gap junctions;    connexins;    human;   

DOI  :  10.1247/csf.18.419

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(30)Cited-By(25)We examined i) the kinds of connexins, component proteins of gap junctions, that are expressed in osteoblasts and ii) the relationship between the expression of gap junctions and osteoblastic phenotype during cell proliferation and after the treatment with 1α, 25-dihydroxyvitamin D3. Human osteoblastic cells with (SV-HFO) or without (HFO) transformation by simian virus 40 and mouse osteoblast-like cells (MC3T3-E1) expressed connexin 43 (Cx43), but not Cx26 or Cx32, as revealed by Northern blot analysis and immunocytochemistry. The expression of Cx43 was significantly higher in SV-HFO cells in the confluent phase than in the proliferative phase. Similarly, the expression of alkaline phosphatase (ALP) and osteocalcin in SV-HFO cells in the confluent phase were higher than those in the proliferative phase. On the other hand, treatment of lα, 25-dihydroxyvitamin D3 did not change the expression of Cx43 in SV-HFO cells, but significantly induced the expression of ALP and osteocalcin. These results showed that the expression of gap junction protein in osteoblastic cells was coupled with cell differentiation in association with the expression of osteoblastic phenotype, but that the connexin expression is regulated in a way different from that of ALP and osteocalcin.

【 授权许可】

Unknown