Proliferation, Migration and Platelet Release by Megakaryocytes in Long-term Bone Marrow Culture in Collagen Gel

Cell Structure and Function

Shigeru Okada²  Masaharu Mori¹  Junjirou Tsuchiyama² 

[1] Faculty of Health and Welfare Science, Okayama Prefectural University;Department of Pathology, Okayama University Medical School

关键词: megakaryocytepoiesis;    collagen gel culture;    capillary;    long-term bone marrow culture;    migration;   

DOI  :  10.1247/csf.18.409

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(30)Cited-By(4)The proliferation of megakaryocytes, their migration, and their platelet release processes were observed in long-term bone marrow culture in collagen gel. Megakaryocytes proliferated for more than 6 months, not only with myeloid cells but also with sinusoid-like capillaries. The megakaryocyte count decreased at 2 weeks of culture, increased to more than 400 at 6 weeks, and then decreased to about 100. Megakaryocyte colonies appeared after 2 weeks of culture; the number increased to more than 10 at 4 weeks of culture, and was maintained at that level. Morphologically, most fully mature megakaryocytes extended several long antennae-like processes, with periodic constrictions, demarcation membranes (DM), and platelet fields. Pro-platelets were released from these processes primarily by a "pinching off" mechanism, but some megakaryocytes released pro-platelets by the dissociation of DM, without extending long processes. Mature megakaryocytes migrated like ameba in collagen gel, and a few migrated to the abluminal side of the capillary or into the capillary; in both cases releasing pro-platelets into the capillary lumina. These observations were very similar to those noted in vivo.

【 授权许可】

Unknown