Cytoskeleton in Human Mammary Carcinoma Cells Forming Three-Dimensional Cellular Structures within Collagen Gels

Cell Structure and Function

Kazuhide Takahashi¹  Tetsuo Ono¹ 

[1] Department of Biochemistry, Kanagawa Cancer Center Research Institute

关键词: morphogenesis;    cytoskeleton;    collagen gel culture;    mammary carcinoma cells;   

DOI  :  10.1247/csf.15.229

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(24)Cited-By(3)Human mammary carcinoma cell line MCF-7 cells grown on type I collagen gels floating in a medium occasionally invaginated into the gels as a cell mass and formed cylindrical or domed structures within it. The 0.05% Triton-insoluble cytoskeleton of such cellular structures sedimented as a white flocculent layer at the boundary between 60 and 70% sucrose layers by ultracentrifugation, and consisted of 4 basal components: 54-kD (β-tubulin), 45-kD, 42-kD (actin), and 39-kD polypeptides. By contrast, the isolated cytoskeleton of MCF-7 cells grown as monolayers on plastic substratum formed a finer cytoskeletal network with a smaller buoyant density and consisted of two distinct polypeptides with apparent molecular sizes of 80-kD and 65-kD in addition to the 4 basal components found in the morphologically developing cells. The present results indicate that the cytoskeleton of MCF-7cells forming the three-dimensional cellular structures within collagen gels is lacking in these two polypeptides, and that it has a coarser cytoskeletal network with a greater buoyant density than that of the monolayeredcells on plastic.

【 授权许可】

Unknown