Correlation of Axenic Linkage Groups with the Position of the Microtubule-Organizing Center in Aggregating Dictyostelium

Cell Structure and Function

Hirotaka Fujimoto¹  Masazumi Sameshima²  Yohichi Hashimoto¹  Yoshihisa Imai¹ 

[1] Department of Physics, Biophysics Laboratory, Rikkyo University;Department of Ultrastructural Research, The Tokyo Metropolitan Institute of Medical Science

关键词: axenic mutation;    cell polarity;    Dictyostelium;    microtubule-organizing center;    nucleus;    rapid-freeze substitution;   

DOI  :  10.1247/csf.17.417

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(40)Cited-By(3)Positioning of the microtubule-organizing center (MTOC)in Dictyostelium discoideum was found to be genetically regulated. We examined the wild-type strain NC-4 cells independently maintained in different laboratories, freshly recovered cells from spores stocked for over 20 years, the temperature-sensitive growth mutant HU49 isolated from NC-4, as well as strain V-12 which is the opposite mating-type to NC-4. During aggregation on nonnutrient agar plates, all these strains showed similar cell polarity, as denned by the alignment of the nucleus ahead of the MTOC. By contrast, in Ax2 and Ax3, axenic strains carrying axenic mutations on linkage groups II and III, the MTOC was usually positioned ahead of the nucleus. Cells containing axenic linkage group II but not III positioned the MTOC ahead of the nucleus. Conversely cell polarity of strains including axenic linkage group III but not II was similar to that of wild-type cells. Thus axenic linkage group II, probably axeC or other linked gene(s) not yet identified, is responsible for the location of the MTOC anterior to the nucleus during aggregation. The anterior positioning of the MTOCs was prevented by growth on bacteria in cells carrying both axenic linkage groups, but not in those carrying only axenic linkage group II.

【 授权许可】

Unknown