The spreading of B lymphocytes induced by CD44 cross-linking requires actin, tubulin, and vimentin rearrangements

Journal of Leukocyte Biology

Adriana Sumoza-Toledo¹  Leopoldo Santos-Argumedo¹ 

[1] Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados, I.P.N., MéxicoDepartment of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados, I.P.N., MéxicoDepartment of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados, I.P.N., México

关键词: cytoskeleton;    hyaluronic acid;    microtubule-organizing center;    motility;   

DOI  :  10.1189/jlb.0803403

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

CD44 is a polymorphic family of adhesion molecules widely distributed on cells and tissues. CD44 is up-regulated on activated lymphocytes, and it can function as a receptor, mediating rolling and migration. Although it has been demonstrated that anti-CD44 antibodies bound to tissue-culture plates induce multidirectional emission of retractile dendrites (“spreading”) in activated murine B lymphocytes, the involvement of cytoskeleton elements in this phenomenon is largely unknown. In this work, it is shown that the generation of dendrites induced by CD44 cross-linking in activated B cells depends on actin, microtubules, and vimentin reorganization. Immunofluorescence analysis showed that dendrite formation began with actin polymerization, and its extension was favored by microtubules and intermediate filaments of vimentin oriented to the polymerized actin. Pretreatment of activated B lymphocytes with cytochalasin E inhibited the dendrites formation; moreover, when cells were treated with this drug at different time points during the dendrite formation process, the stability of the dendrites was affected. In contrast, although the treatment with colchicine and nocodazole (tubulin polymerization inhibitors) inhibited the dendrites formation, it did not inhibit the initial phase of actin polymerization. According to these results, B cell spreading and dendrite formation induced by anti-CD44 antibodies require coordinated rearrangements of actin, microtubules, and vimentin, being the actin cytoskeleton, the most important element that confers stability and drives the morphological changes during B cell spreading, conceivably preparing B lymphocytes for locomotion.

【 授权许可】

Unknown