Establishment of Mouse Oligodendrocyte/Type-2 Astrocyte Lineage Cell Line by Transfection with Origin-Defective Simian Virus 40 DNA

Cell Structure and Function

Akio Suzumura⁴  Akira Takahashi³  Tohru Marunouchi¹  Makoto Sawada¹  Izumi Nakashima²  Kunihiko Ohtani³ 

[1] Division of Cell Biology, Institute for Comprehensive Medical Science;Department of Immunology, Nagoya University School of Medicine;Department of Neurology, Nagoya University School of Medicine;Department of Neurology, School of Medicine, Fujita Health University

关键词: oligodendrocyte;    type-2 astrocyte;    glial precursor;    simian virus 40 (SV40);    transfection;    differentiation;   

DOI  :  10.1247/csf.17.325

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(44)Cited-By(12)A permanent glial cell line has been established from the neonatal mouse primary mixed glial cell cultures by transfection with replication origin-defective simian virus 40 DNA. This cell line, designated OS3, has morphological similarity to type-2 astrocyte and expresses an astrocyte-specific marker, glial fibrillary acidic protein (GFAP), when cultured in the presence of 10% calf serum (CS). OS3 cells do not express the O4 antigen, galactocerebroside (GalC) and A2B5 under this culture condition. When cultured in a medium containing 2% CS or a chemically defined medium, these cells undergo morphological transformation. Someof these cells express O4 antigen and/or GalC, and the percentage of GFAP positive cells decreases under these conditions. Thus depending on the culture conditions, the OS3 cells display either type-2 astrocyte properties or immature oligodendrocyte characteristics. Furthermore, the OS3 cells show similar responses to the various growth factors as do oligodendrocyte/type-2 astrocyte (O-2A) progenitors. Therefore, the OS3 cell line is an unique mouse bipotential permanent O-2A lineage cell line which may be useful to analyze the developmental properties of these glial cells.

【 授权许可】

Unknown