Effects of Fibronectin-Type V Collagen Recombinant Fusion Protein on Cell Adhesion and Cell Proliferation

Cell Structure and Function

Ikunoshin Kato²  Mika Hatai¹  Yoshihito Yaoi¹  Hidetaka Hashi²  Kazuhiko Takahara² 

[1] Biology Division, National Cancer Center Research Institute;Biotechnology Research Laboratories, Takara Shuzo Co.

关键词: fibronectin;    type V collagen;    cell adhesion;    insulin;   

DOI  :  10.1247/csf.17.293

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(15)Cited-By(4)An expression vector pTF7520-Col-V-In, which encodes a fusion protein of the cell-binding domain of fibronectin (C277) and the insulin- and heparin-binding domain of the α1 chain of human type V collagen, was constructed. E. coli transfected with this plasmid synthesized a 50-kDa fusion protein. This fusion protein, C277-V, was purified from the crude extract by a single step heparin HPLC.Similar amounts of insulin bound to purified C277-V and to theα1 chain of type V collagen as judged by the binding of peroxidase-conjugated insulin. Cell-adhesive activity of C277-V was lower than that of the original fibronectin fragment C274, but similar numbers of cells adhered to both protein substrates when the culture dishes were coated with 1 mM of each protein. Insulin bound to the C277-V substratum stimulated the growth of mouse mammary tumor MTD cells in serum-free culture medium.

【 授权许可】

Unknown