Chemical Modification of Recombinant Human Granulocyte Colony-Stimulating Factor by Polyethylene Glycol Increases its Biological Activity in vivo

Cell Structure and Function

Mayumi Kawagishi¹  Rika Satake-Ishikawa¹  Katsuhiko Asano¹  Masaharu Ishikawa¹  Makoto Kakitani¹  Shigeru Matsuki¹  Yuji Okada¹ 

[1] Pharmaceutical Laboratory, Kirin Brewery Co., Ltd.

关键词: granulocyte-colony stimulating factor;    polyethylene glycol;    chemical modification;   

DOI  :  10.1247/csf.17.157

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(16)Cited-By(14)Recombinant human granulocyte colony-stimulating factor (rHuG-CSF) produced in Escherichia coil was chemically modified by polyethylene glycol (PEG) of molecular weights 4, 500 or 10, 000. The neutrophils observed at 32 hours after intravenous injection of the rHuG-CSF modified with PEG (4, 500) or PEG (10, 000) to mice were, respectively, 2.5 times and 5 times more than that observed after the injection of the un-modified rHuG-CSF. These results show that the covalent attachment of PEG to rHuG-CSF enhanced its pharmacological activity in vivo and that the modification with the larger PEG molecule is more effective to enhance the in vivo activity of rHuG-CSF.

【 授权许可】

Unknown