Isolation of mononuclear cells of peripheral blood for stem cell transplant: Simplified method

Revista Cubana de Hematología, Inmunología y Hemoterapia

Cortina Rosales, Lázaro¹  Matamoros, María de los Ángeles¹  Artaza Sanz, Heriberto M¹  Dorticós Balea, Elvira¹  Lam Díaz, Rosa María¹  Macías Abraham, Consuelo¹  Salgado Arocena, Odalis¹  Socarrás Ferrer, Berta B¹  González Iglesias, Ana Iris¹  Hernández Rego, Yakima¹  González Suárez, Tania¹  López De Roux, Maríadel Rosario¹  Hernández Ramírez, Porfirio¹ 

[1] Instituto de Hematología e Immunología, Ciudad de La Habana, Cuba

关键词: stem cells;    granulocyte-colony stimulating factor;    mononuclear cells;    hydroxyethyl starch;   

DOI  :  

学科分类：生理学与病理学

来源: SciELO

【 摘 要 】

In the last years, the topic of stem cells has arisen an increasing interest for its therapeutic potential in diseases that have not an effective treatment so far. A prospsective and exploratory study was conducted in patients with obliterant atherosclerosis of the lower limbs to evaluate the safety and effectiveness of a manual method of collection and processing of mononuclear cells and of CD34+ cells, starting from mobilized peripheral blood. The blood was processed in closed collection systems, using hydroxyethyl starch as a potentiator of erythrocyte sedimentation. The patients were treated with granulocyte colony stimulating factor at total doses of 40µcg/kg of weight during 2 days. Self-donation was performed when the leukocyte count was higher than 20 x 109/L. To assess the efficacy of the method, the amounts of nucleated cells, of mononuclear cells and of CD 34+ cells in the cellular concentrate were analyzed. Cellular viability was determined and a microbiological study of the material obtained was conducted. It was proved that the method is efficient and safe, since adequate cellular levels with a high viability and absence of bacterial contamination are attained. On the other hand, it is simple and cheap, which allows its application in other health centres, particularly in those with less resources. This makes possible that more patients benefit from the stem cell treatment.

【 授权许可】

CC BY-NC