Intracellular Localization and Partial Amino Acid Sequence of a Stress-Inducible 40-kDa Protein in HeLa Cells

Cell Structure and Function

Kenzo Ohtsuka²  Ying-Chun Liu⁴  Minoru Ueda⁴  Tatsuya Kobayashi³  Iwai Tohnai⁴  Toshio Kaneda⁴  Kazushi Tanabe¹  Hirotomo Hattori⁴ 

[1] Biophysics Unit, Aichi Cancer Center Research Institute;Laboratory of Experimental Radiology, Aichi Cancer Center Research Institute;Department of Neurosurgery, Komaki City Hospital;Department of Oral Surgery, Nagoya University School of Medicine

关键词: 40-kDa stress protein;    heat shock;    DnaJ homologue;    intracellular localization;    HeLa cells;   

DOI  :  10.1247/csf.17.77

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(40)Cited-By(29)We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalianand avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal amino acid sequence of this protein. The hsp40 is homologous to DnaJ, an Escherichia coli heat-shock protein, as well as to DnaJ-homologous proteins in yeast such as SCJ1, Sec63/Np11, YDJ1 and SIS1. Indirect immunofluorescence staining using an anti-hsp40 polyclonal antibody demonstrated that hsp40 was localized faintly throughout the cell in non-heat-shocked cells and was accumulated in nuclei and nucleoli in heat-shocked cells. The intracellular localization of hsp40 was very similar to that of hsp70, suggesting that these two hsps colocalize in heat-shocked HeLa cells.

【 授权许可】

Unknown