Expression of Chicken Integrin β1 Subunit in Rat PC12 Cells

Cell Structure and Function

Takuji Kawashima²  Barb Reinish³  Alfred Reszka³  Alan F. Horwitz³  Taisen Iguchi¹  Yokichi Hayashi² 

[1] Department of Biology, Yokohama City University;Biochemical Research Institute, Morinaga Milk Ind. Co. LTD;Department of Cell and Structural Biology, University of Illinois

关键词: Integrin β1 subunit;    Chimeric receptor;    PC12 cells;    Cell spreading;   

DOI  :  10.1247/csf.16.135

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(15)Cited-By(2)We transfected rat pheochromocytoma (PC12) cells with a cDNA encoding chicken integrin β1 subunit. The chicken integrin β1 subunit produced in stable transfectants associated with two major α subunits of rat integrins to form interspecific chimeric receptors. These receptors mediated cell spreading and initial neurite outgrowth on laminin as did corresponding endogenous integrins, although they were slightly less effective in inducing cell adhesion to laminin. These results indicate that chicken integrin β1 may functionally substitute for β1 subunit of rat integrins in PC12 cells. Apparently, the structure of the integrin β1 subunit is highly conserved in the evolution of these species.

【 授权许可】

Unknown